Journal: International Journal of Molecular Sciences
Article Title: Mitoregulin Promotes Cell Cycle Progression in Non-Small Cell Lung Cancer Cells
doi: 10.3390/ijms26051939
Figure Lengend Snippet: MTLN silencing decreases endogenous H 2 O 2 levels and reduces sensitivity to exogenous H 2 O 2 toxicity. ( A ) The cytosol- and mitochondria-localized roGFP2-Orp1 H 2 O 2 sensors are less oxidized in siMTLN compared to siCont cultures. Bars show mean ± SD ( n = 3) ** p < 0.01, *** p < 0.001 by unpaired t -test. ( B ) Distinct dose–response effects of H 2 O 2 pulse on cell proliferation in siMTLN compared to siCont cultures. Cultures were exposed to the indicated amount of H 2 O 2 for 3 h and then harvested and counted 24 h later. Bars show mean ± SD ( n = 4) * p < 0.05, **** p < 0.0001 by 2-way ANOVA with multiple comparisons.
Article Snippet: Cytosol- and mitochondria-localized roGFP2-Orp1 H 2 O 2 sensors were subcloned from plasmids donated to Addgene from Tobias Dick [ ] (Addgene plasmids #64991 and #64992, Watertown, MA, USA) into a VVC adenovirus shuttle vector (VVC G0688; pacAd5CMVmcspA) using standard cloning techniques, and vector particles (Adcyto-roGFP2-Orp1 and Admito-roGFP2-Orp1) were generated by the VVC.
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